Semiautomated assay of normal concentrations of urinary glucose by an enzymatic fluorometric technic.

A semiautomated, enzymatic, fluorometric method for the determination of the low glucose concentrations normally present in urine from subjects in the fasting state is described. The method is developed from a manual, enzymatic, fluorometric technic for the determination of urinary glucose using hexokinase (HK) and glucose-6phosphate dehydrogenase (G-6-PDH). The procedure is based on measurements of the fluorescence of reduced nicotine amide adenine dinucleotide-phosphate (NADPH2) which is formed in proportion to the glucose content of the sample. The unspecific fluorescence of urine is almost completely removed by treating the urine with a mixed ion-exchange resin prior to applying the automated procedure. The chief advantage of the method is that it may afford an opportunity for low-cost screening for detection of bacteriuria.